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KMID : 0545120040140010178
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Journal of Microbiology and Biotechnology
2004 Volume.14 No. 1 p.178 ~ p.181
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Molecular Cloning, Purification, and Characterization of an Extracellular Nuclease from Aeromonas hydrophila ATCC14715
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NAM, IN-YOUNG
MYUNG, HEEJOON/JOH, KISEONG
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Abstract
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A gene encoding an extracellular nuclease was cloned from Aeromonas hydrophila strain ATCC147 15. The gene was overexpressed and the enzyme was purified by fusing to maltose binding protein. It was shown that the protein possessed DNase activity on both single-stranded and double-stranded DNAs. It exhibited both endo- and exonuclease activities. It was also shown that the protein had an RNase activity. Possible roles of this extracellular enzyme in the A. hydrophila life cycle are discussed.
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KEYWORD
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